Annie Killoran1* and Kevin Biglan2
1Department of Neurology, University of Iowa, IA, USA
2Department of Neurology, University of Rochester, New York, USA
Huntington’s disease (HD) causes progressive neurological deterioration that leads to death. It is inherited in an autosomal dominant fashion, and individuals with a positive family history can be tested for the presence of the HD mutation prior to the development of the overt features that subjectively define disease onset. An objective biomarker denoting this time point would improve onset accuracy, and ideally be sufficiently sensitive to monitor progression leading up to this juncture. Once manifestations arise, patients are treated symptomatically. There are no disease-modifying treatments available for HD, but many are in development. A major goal is to develop a therapy that will delay the onset of the disease or to potentially even prevent the disease from occurring altogether. However, how does one assess the efficacy of these experimental therapeutics in individuals who carry the HD gene mutation, but are clinically-unaffected? Sensitive and reliable outcome measures are required for preventative clinical trials. Candidate biomarkers include subtle, but quantifiable abnormalities detected on clinical exam, findings on brain imaging, and levels of pathologically-relevant molecules collected in bodily fluids.
Huntington’s disease (HD) is an autosomal dominant, neurodegenerative disorder, typically presenting in young middle-age. It is characterized by prominent psychiatric problems, as well as progressive deterioration in both cognitive function and motor control, leading to an early death. HD is caused by an expanded trinucleotide cytosine-adenine-guanine (CAG) repeat in the huntingtin gene1. The knowledge of this gene mutation has resulted in HD being one of the rare neurodegenerative conditions for which predictive genetic testing is available for individuals with a known family history. This enables identification of HD gene mutation carriers while they are still healthy, before developing overt clinical features of the disease. These ‘pre-manifest’ individuals are uniquely poised to potentially benefit from neuroprotective therapy, which would delay (or even prevent) the development of the disease’s clinical manifestations and functional disability. Currently no such disease-modifying therapies are available, and HD is managed with limited, only partially-effective, symptomatic treatments. However, with the growing knowledge of the underlying pathobiology of HD, new therapies are being developed.
Therapeutic clinical trials aiming to evaluate the efficacy of potential disease-modifying treatments during pre-manifest HD requires biomarkers to serve as outcome measures. Suitable ‘pharmacological’ or ‘efficacy’ biomarkers’ are those that reliably and objectively respond to treatment in a predictable manner. Some efficacy biomarkers may also function as ‘state biomarkers’ or ‘biomarkers of progression’, which are used as indications of disease severity. Ideally, these state biomarkers reflect the underlying disease pathobiology and linearly track clinical progression throughout the disease (including during the pre-manifest stage). Though cross-sectional studies are helpful in identifying potential markers whose levels correspond to disease stage, the relationship between these candidate markers and disease progression is best evaluated by tracking results from longitudinal studies2. All biomarkers should be affordable, easily accessible, unaffected by comorbidities, and have limited variability amongst the general population3. In addition, biomarker sampling and testing should be standardized to minimize variation between facilities4. Table 1 includes candidate state biomarkers that have shown an association with stages of disease severity in both pre-manifest & manifest HD in humans.
Biomarker Source |
Feature | Test | Comments |
---|---|---|---|
Clinical | Motor deficit | Anti-saccade error rate10 | Cross-sectional study; correlated with disease severity according to 4 groups (early and late pre-manifest and 2 groups of early (stage 1 and 2) HD; early pre-manifest did not differ from controls |
Digitomotography8 | Large cohort; longitudinal study (36 months) | ||
Cognitive deficit | SDMT8 | ||
Imaging | Structural loss | MRI8 | Large cohort; longitudinal (36 months) |
PDE10 uptake | [18F]MNI-659 PET45 | Small cross-sectional study (8 early HD and 3 pre-manifest HD) | |
Wet: blood | Immune system | IL-846 | Cross-sectional study; correlated with disease severity according to 3 groups (pre-manifest, early and moderate HD) |
TNF-α46 | |||
Neuro-degeneration | NfL47 | Only 3 pre-manifest included; not HD-specific |
Table 1: Summary of candidate HD state biomarkers which associated with disease severity in pre-manifest and manifest HD
Abbreviations: HD, Huntington’s Disease; SDMT, Symbol Digit Modalities test; MRI, Magnetic resonance imaging; PDE10, phosphodiesterase 10A; [18F]MNI-659 PET, 2-(2-(3-(4-(2-[18F]fluoroethoxy)phenyl)-7-methyl-4-oxo-3,4-dihydroquinazolin-2-yl)ethyl)-4-isopropoxyisoindoline-1,3-dione positron emission tomography; IL-8, Interleukin 8; TNF-α, Tumor Necrosis Factor-alpha; NfL, neurofilament light chainCurrently, the United Huntington's Disease Rating Scale (UHDRS) is used to assess for treatment response in therapeutic trials in HD, as well as to monitor disease progression. It is a collection of scales that was designed to detect clinical changes in manifest HD. As such, the UHDRS may not be sensitive enough to detect the subtle features seen in some pre-manifest individuals, particularly those who are several years away from developing the disease5. The UHDRS includes Diagnostic Confidence Level Criteria, which are used in trials to denote the clinical onset of HD. This dichotomous time point occurs when there are sufficient motor abnormalities present on physical examination, such that the examiner is 99% confident that the individual has HD. However, the interrater reliability amongst clinicians is considered only adequate, (with a weighted kappa = 0.67, SE = 0.09)6. In addition, HD patients’ motor features are not a reliably constant measure, e.g. they typically worsen with stress. The subjective designation of disease onset can also seem somewhat arbitrary, given that the disease has a lengthy prodrome that includes various subtle motor and cognitive abnormalities that develop insidiously over several years7,8. The use of disease onset as an end point in clinical trials would therefore require following a large pre-manifest study population for many years, making it somewhat unrealistic7,8. An objectively measured state biomarker is needed to replace this subjective, motor-centric time-point, as well as for monitoring the severity of disease in pre-manifest individuals. Currently, pre-manifest trial subjects are staged according to the predicted duration of time until their expected disease onset; this is calculated with a non-standardized estimation using a patient’s age and CAG repeat length.
Rather than relying on the UHDRS’s dichotomous notion of ‘disease onset’, some researchers have proposed the use of continuous measures9, such as clinical features. Some UHDRS motor abnormalities can be objectively quantified, thereby improving accuracy and reducing inter-rater and intra-rater variability. For example, a modular force transducer arrangement can be used to quantify finger tapping precision, (digitomotography)10. Deficits in this task are evident even in pre-manifest HD and worsen over time, correlating with other clinical features11,12. Subtle cognitive impairment can also be detected in pre-manifest subjects. One of the more reliable measures has been the Symbol Digit Modalities test8,13, which assesses visual attention and psychomotor speed. However, such clinical markers have not proven sufficient sensitivity to subtle changes over time during the pre-manifest period14,15, so their value as outcome measures for use in preventative clinical trials is limited. In addition to this ceiling effect in pre-manifest HD, clinical biomarkers are also susceptible to floor effects in advanced disease. Plus, they can vary according to subjects’ state of being, and may be confounded by the retest effect, education level and potential language barriers in non-native speakers3. Finally, relying on clinical biomarkers has the added challenge of trying to distinguish between a treatment’s symptomatic improvement vs underlying disease modification3.
In HD trials, clinical outcome measures are typically used in combination with brain imaging, which has proven to be a more robust marker during the pre-manifest period14. For example, in the PRECREST study, the rate of cortical thinning was significantly reduced at 6 and 18 months in subjects who were at risk for HD on creatine treatment14. This is despite there being no detectable clinical change in the study subjects, which is not unexpected given the scarcity of clinical examination findings at this prodromal stage of disease. Ultimately one would want to validate the imaging findings against the cohort’s future clinical outcomes. Using brain atrophy as an efficacy measure can be limited by the typically slow rate at which it occurs, making it somewhat impractical for most clinical trials. Compared to structural imaging, functional and metabolic imaging techniques may be more sensitive to change over short periods of time. These techniques may also allow for some interesting visualization of potentially-reversible findings that match nicely with clinical features2. For example, compared to riluzole-treated HD subjects, those treated with placebo had reduced metabolic FDG uptake in the parietal lobe that linearly correlated with worsening motor scores. Whereas reduced uptake in the frontal and temporal areas correlated with worsening behavioral measures16.
Structural imaging has shown utility in monitoring disease progression in the very early pre-manifest stage. Volume loss, notably in the striatum, is detectable 1-2 decades prior to the development of motor features17-19. The atrophy progresses over time18 and correlates with disease load20. This supports the use of structural imaging as a state biomarker, though it is limited in that it does not provide a direct pathological measure of disease.
Wet biomarkers, (those obtained from bodily fluids), are another potential source of useful outcome measures, particularly if they reflect the disease’s pathobiology. Various pathologic mechanisms have been implicated in HD, yielding numerous potential molecular markers.
For example, the pathogenesis of HD seems to involve the dysregulation of gene transcription through post-translational modifications of histones. H2AFY is a particular histone that is elevated in individuals with HD21, though it does not track disease progression, making it unsuitable as a state biomarker. However, it has shown promise as a potential therapeutic marker. In subjects with early HD, H2AFY levels responded to treatment with Sodium Phenylbutyrate, a histone deacetylase (HDAC) inhibitor, which is known to suppress neurodegeneration in mouse models of HD21.
8-hydroxy-2-deoxyguanosine (8-OHdG) is a measure of oxidative stress22, which is believed to be involved in the pathogenesis of HD23. As a hopeful candidate biomarker24, it was the focus of numerous studies. Elevated 8-OHdG was detected in HD subjects’ brain and blood in several studies25-29, (though not all)30, and levels correlated with proximity to projected disease onset31. Unfortunately, larger investigations found no association with disease severity10,32, making it unsuitable as a state biomarker. However, 8-OHdG also showed promise as an efficacy biomarker in antioxidant trials33,34, including one in a murine model of HD, in which there was significant improvement35. In human HD studies, 8-OHdG levels also decreased following treatment with the antioxidants creatine27, and Coenzyme Q10(CoQ)30, but it remained unchanged in a subsequent, pre-manifest trial of CoQ36, refuting its reliability. 8-OHdG also lacked HD-specificity, which is a common short-coming amongst candidate biomarkers. For example, serum titers of the inflammatory marker angiotensin II type 1 receptors (AT1R) antibody correlated with the UHDRS motor score, as well as with smoking and concurrent infection, thus making results potentially misleading37.
One potential wet biomarker being studied that is presumably HD-specific is the mutant Huntingtin protein (mHtt). This is an attractive candidate given that it is the principal pathogenic molecule of HD. In addition, besides being detected in CSF38,39 and blood40, it is also measurable in saliva41, providing convenient, non-invasive access to a protein whose damage is focused on the central nervous system. In HD gene carriers, peripheral immune cell levels of mHTT are elevated, with differences in mean mHTT between pre-manifest and early-stage HD subjects, but not between early-stage and moderate-stage subjects40. Levels of mHtt also associate with disease burden scores and caudate atrophy rates in gene positive individuals40. One study found CSF mHtt levels associated with motor and cognitive features in pre-manifest and early-mid HD, but not in late HD38. However, an earlier study demonstrated its correlation with UHDRS motor scores in individuals at stages ranging from pre-manifest to advanced disease42.
MHtt has yet to be tried as an efficacy biomarker in humans, but in animal studies it has shown utility as a pharmacologic marker of nucleotide-based gene silencing therapies aimed at lowering mHtt. Both antisense oligonucleotides (ASO) and RNA interference (RNAi) reduced levels of mHtt in the striatum and CSF, which in turn corresponded with significant phenotypic improvements43,44. In humans, the first gene silencing trial in HD is currently underway, (NCT02519036). It is evaluating an antisense oligonucleotide that targets mutant Huntingtin RNA, which is expected to result in reduced mHtt production and therefore less of the neuronal destruction that is characteristic of HD. Naturally, one of the study’s outcome measures is mHtt, functioning as an efficacy biomarker.
The availability of predictive genetic testing has enabled for large observational trials of pre-manifest individuals for the characterization of their insidiously developing clinical features. Subjects are staged by the amount of time that is predicted for them to reach the subjective and motorically-defined disease onset. A reliable state biomarker (or set of biomarkers) that is sufficiently sensitive for use in the pre-manifest stage is needed for marking progression during this early part of the disease, as well as for a more accurately defined ‘onset’.
Predictive genetic testing has also made HD the ideal disorder for which to develop a neuroprotective treatment, with the goal of delaying or even preventing the clinical features of the disease. The ability to assess the therapeutic response to experimental drugs is a major challenge at the pre-manifest stage. Research efforts are currently geared towards finding reliable and objective biomarkers to suit this purpose. A range of clinical abnormalities are detectable in pre-manifest HD, but with limited sensitivity to change over time. Wet biomarkers that reflect the pathobiology of HD are logical candidates being explored. Current hope lies in pathologically-relevant molecules such as mHtt, most likely in combination with other quantifiable markers, notably measurable changes detected on brain imaging.
Kevin Biglan has served as a consultant for KJT and UCB and has received research funding from AMC Health, Michael J. Fox Foundation, National Institute of Neurological Disorders and Stroke, National Parkinson Foundation, Lundbeck, Huntington’s Disease Society of America, Patient-Centered Outcomes Research Institute, and Greater Rochester Health Foundation.